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Anti-Clathrin heavy chain antibody [TD.1] (ab24578)反網(wǎng)絡(luò)蛋白重鏈抗體產(chǎn)品信息：
- Product nameAnti-Clathrin heavy chain antibody [TD.1]See all Clathrin heavy chain primary antibodies ...
- Description
Mouse monoclonal [TD.1] to Clathrin heavy chain
- SpecificityThis antibody reacts with clathrin heavy chains (CHC).
- Tested applicationsIHC-P, WB, ICC/IF
- Species reactivity
Reacts with: Cow, Human
- Immunogen
This antibody was raised using the globular N terminal domain of clathrin heavy chain.
- Positive control
- Methanol fixed HeLa cells for IF.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.03% Thimerosal (merthiolate).
Constituents: PBS. Concentration 100 µl at 1 mg/ml
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberTD.1
- IsotypeIgG1
- Research Areas
- Signal Transduction
- Protein Trafficking
- Vesicle Transport
- Coat Proteins
- Tags & Cell Markers
- Subcellular Markers
- Organelles
- Caveolae and Clathrin
Applications
Our Abpromise guarantee covers the use of ab24578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use at an assay dependent concentration. PubMed: 21571834 |
| WB | WB: 1/250. Predicted molecular weight: 192 kDa. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
- Application notesIs unsuitable for or IP.
Target
- FunctionClathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
- Sequence similaritiesBelongs to the clathrin heavy chain family.
- Cellular localizationCytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
Database links
- Entrez Gene: 281080 Cow
- Entrez Gene: 1213 Human
- Omim: 118955 Human
- SwissProt: P49951 Cow
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
- Unigene: 491351 Human
Alternative names
- CHC17 antibody
- Clathrin heavy chain 1 antibody
- Clathrin heavy chain 2 antibody
Anti-Clathrin heavy chain antibody [TD.1] images
![Immunocytochemistry/ Immunofluorescence - Clathrin heavy chain antibody [TD.1] (ab24578)](http://a.abcam.com/ps/datasheet/Images/24/ab24578/ab24578_1.jpg)
Immunocytochemistry/ Immunofluorescence - Clathrin heavy chain antibody [TD.1] (ab24578)
ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.
公司介紹：
Abcam位于英國(guó)的劍橋科學(xué)園，成立于1998年，專門生產(chǎn)和分銷研究型抗體。主要?jiǎng)?chuàng)立人Jonathan Milner和實(shí)驗(yàn)室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司，理念是Abcam銷售質(zhì)量高的抗體，并提供zui全面、誠(chéng)實(shí)、時(shí)新的說明書，快速的送貨服務(wù)，及高效率的顧客和服務(wù)。我們的在線目錄(www.myshuxun.com) 已有差不多100,000種抗體和試劑，并不斷添加，供應(yīng)予百多個(gè)國(guó)家。Abcam于2005年11月在倫敦證券交易所上市，在美國(guó)馬薩諸塞州、日本東京及香港均設(shè)有分公司。
Abcam 一直致力加強(qiáng)產(chǎn)品線，為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品，在2011并購(gòu)了美國(guó)的MitoSciences公司，加強(qiáng)了免疫分析方面的產(chǎn)品供應(yīng)；同年也并購(gòu)了英國(guó)的Ascent Scientific 公司，開展了生化試劑的供應(yīng)。在2012年，Abcam并購(gòu)了美國(guó)的Epitomics 公司，成為一家有的RabMAbs® 供應(yīng)商。
Abcam 的 目標(biāo)是給世界上的抗體建立zui大的在線目錄，為各地科學(xué)家提供*產(chǎn)品，成為各國(guó)科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來使客戶獲得預(yù)期的結(jié) 果。為提供高質(zhì)量的抗體來指向盡可能多的靶蛋白，我們盡所能在盡可能多的應(yīng)用和物種中檢測(cè)每種抗體。我們相信誠(chéng)信才是上策，在我們的上會(huì)發(fā)布有關(guān)我們 每一種產(chǎn)品的盡可能多的信息。
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Abcam ab12616Rabbit polyclonal to GPCR TGR750ug3996GPCR LS195564hystemcell一抗
Abcam ab1262Mouse monoclonal [GA90] to CD45500ul3996CD45hystemcell一抗
Abcam ab12623Rabbit polyclonal to IL3 （Biotin）25ug3996IL-3hystemcell一抗
Abcam ab1263Goat polyclonal to Myc tag （FITC）100ug3996Myc taghystemcell一抗
Abcam ab12631HIF Prolyl Hydroxylases panel1kit7049HIF Prolyl Hydroxylases panel試劑盒及其他產(chǎn)品試劑盒
Abcam ab1264Mouse IgG1 （HybIgG1） （FITC） - Isotype control1ml3996Mouse IgG1hystemcell同型對(duì)照
Abcam ab1265Goat polyclonal to HA tag （HRP）100ug3996HA taghystemcell一抗
Abcam ab12650Rabbit polyclonal to IL16 （Biotin）25ug3996IL-16hystemcell一抗
Abcam ab12656Rabbit polyclonal to IP10 （Biotin）25ug3996IP10hystemcell一抗
Abcam ab1266Goat polyclonal to HA tag （FITC）250ug3996HA taghystemcell一抗
Abcam ab1267Goat polyclonal to Glu-Glu tag （HRP）100ug3996Glu-Glu taghystemcell一抗
Abcam ab12675Rabbit polyclonal to GPCR MCH250ug3996GPCR MCH2hystemcell一抗
Abcam ab12678Rabbit polyclonal to GPR 17950ug4940GPCR AGR9hystemcell一抗
Abcam ab1268Goat polyclonal to Glu-Glu tag （FITC）250ug3996Glu-Glu taghystemcell一抗
Abcam ab12680Rabbit polyclonal to D650ug3996GPCR D6hystemcell一抗
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